Competitive Bead-Based Fluorescence Assay to Quantify Antibody Concentration

The aim of cell line development is to create cell lines that are robust, show optimal growth and produce high titers of proteins such as monoclonal antibodies. Traditionally the high performing cells of such a clone library had to be identified using methods such as Western Blot or ELISA. Both methods are very time consuming and need lots of materials as well as hands-on time. To overcome these drawbacks the company PAIA Biotech has created a bead-based assay that allows the quantification of cell line secreted protein in a high throughput format. This application note demonstrates how three different monoclonal antibodies were successfully measured in the CLARIOstar® microplate reader from BMG LABTECH.