Comprehensive Characterization of Trastuzumab using a Single-Shot Peptide Mapping Approach with CESI-MS

Capillary electrophoresis (CE) has exceptional separation efficiency and capabilities for peptide mapping of mAbs by mass spectrometry. Technology involving an electrospray ionization (ESI) emitter integrated with capillary electrophoresis (CE) has been developed, combining CE separation and ESI into a single dynamic process (CESI). This poster presents data for the bottom-up proteomic peptide mapping of a leading, representative monoclonal antibody, Trastuzumab (Herceptin) using a single protease, trypsin. A Beckman Coulter CESI 8000 system sold through SCIEX Separations, a part of AB SCIEX, coupled to a Thermo Q-Exactive was used for the analysis.