Comprehensive Comparison of Protein Microarray Supports using Contact and Non-Contact Systems

The quality of biochips depends on both the support membrane and the deposition technology. This application note compares Nitrocellulose Membranes (NC) Sartorius white (SCHOTT Nexterion Slides NC-W) and Sartorius black (SCHOTT Nexterion Slides NC-D*), to other comerically available NC slides. The probe deposition was performed using a split-pin spotter (Genetix Qarray mini), pin and ring spotter (Affymetrix 417 Arrayer) and a non-contact spotter (sciFlex Arrayer S3).