Corning® HYPERFlask® Cell Culture Vessel jetPEI™ Transfection Protocol

One of the most useful tools in cell biology research is transfection, the introduction of foreign DNA into eukaryotic cells. In much of today’s research, there is a growing need for the effective transfection of large quantities of cells. Polyplus-transfection’s jetPEI transfection reagent is a highly efficient, low toxicity, water-soluble polymer that can be used in the presence of serum in culture media. Therefore, there is no need to change the culture medium before or after transfecting cells, making this method ideally suited for use with the Corning HYPERFlask cell culture vessel. This protocol was optimized using HeLa cells but has been successfully applied to a variety of cell types including Chinese hamster ovary (CHO) cells. This protocol is intended as a starting point that can be optimized by the end user for their cell lines.