CRISPR/Cas9 genome-edited cells express nanoBRET-donor that monitors protein interaction and trafficking

In this application note, BMG LABTECH show how a novel CRISPR/Cas9 technique successfully fused the Nluc BRET donor to endogenously-expressed CXCR4. Luminescence generated by the resulting protein luciferase fusion was sufficient to monitor receptor protein interactions as well as trafficking. The multiplex internalization assay depends on two acceptor fluorophores whose selective detection was rendered possible by the CLARIOstar’s monochromator.