Cross-competition or epitope binning assays on the Octet HTX system

Epitope binning is a term used to describe segmentation of a panel of monoclonal antibodies (mAbs) into bins based upon the antigen region, or epitope, bound by each antibody. This grouping is performed using cross-competition assays, in which the competitive binding of antibody pairs to a specific antigen is characterized. The Octet HTX system is a powerful platform to perform high-throughput binning assays in a matter of a few hours. In this application note, ForteBio shows how the Octet HTX system is ideally suited to performing epitope binning/cross-competition assay due to its high-throughput and powerful software features.