Cryo-Confocal Imaging with Airyscan Improving Resolution and Signal-to-Noise in Cryo-Fluorescence Microscopy

A major limitation for correlative cryo-fluorescence microscopy is the current unavailability of cryo-immersion optics that could yield a higher numerical aperture. In this application note, combining ZEISS LSM 880 with Airyscan and cryo-fluorescent imaging, allowed data to be obtained with a significant increase in resolution and signal-to-noise compared to standard confocal images under cryoconditions.