Detection of Amino Acids Using HPLC with CD Detection

This application note will describe chiral detection of amino acids using HPLC. Amino acids are often described as the “building blocks” of the body. The twenty naturally occurring amino acids that comprise proteins are almost all of the L- form. The L (Levorotatory) form is the stereoisomer that rotates plane polarized light to the left. One method to detect amino acids and determine their stereochemistry is with HPLC.