Detection of Fluorescence in Transgenic Arabidopsis Lines ExpressingFluorophores in Different Subcellular Localizations

The usage of fluorophores such as YFP and GFP is a widely distributed and established method in plant research. To monitor fluorescent reporter proteins several techniques based on instruments such as fluorescent microscopes or imaging systems are available. Detection of free or fused fluorescent reporters in different subcellular localizations and with different expression levels can be performed with the NightSHADE LB985 imaging system. The obtained results, presented in this application note, correlated with data received by detection of fluorescent using the Mithras LB940 multimode plate reader.