Detection of Human Fc Fusion Proteins with Cyto-Mine

The Cyto-Cellect^® Fc Fusion Detection Kit, optimized for use with the Cyto-Mine^® Single Cell Analysis Platform, enables precise detection and selection of high-productivity Fc fusion protein-secreting cells through a FRET-based assay. This advanced tool streamlines single-cell screening and cell line development while maintaining an animal-origin-free workflow.

This application note highlights the kit’s effectiveness in detecting Fc fusion proteins with human IgG Fc domains. It demonstrates the kit’s compatibility with plate readers for reliable quantification of human Fc fusion proteins and IgG subclasses (IgG1-4). Additionally, in Cyto-Mine^®, the kit successfully facilitated the isolation of Fc fusion protein-secreting cells, including those producing IgG4κ.