Detection of Murine Regulatory T Cells on the Attune™ NxT Flow Cytometer

This application note describes the use of the Attune NxT Flow Cytometer for 3-color immunophenotyping analysis of stained mouse splenocytes using the Foxp3 Transcription Factor Staining Buffer Kit, a fixation and permeabilization kit designed to work with transcription factors, like Foxp3, and other intracellular markers. Regulatory T cells come in many forms, with the most understood being those that express CD4, CD25, and Foxp3 (CD4+ CD25+ regulatory T cells). CD4+ CD25+ regulatory T cells are a specialized subpopulation of T cells that act to maintain homeostasis within the immune system by suppressing the immune response of other cells. This is an important “self-check” built into the immune system to prevent excessive reactions.