Detection of Phospho-Akt (Ser 473) as an Indication of PI3K Pathway Activation in MCF7 Cells

The Phosphoinositide 3-kinase (PI3K) pathway has been studied extensively for its role in the development of human cancers. Tools to detect the regulatory impact of PI3K inhibitors on pathway activation have become essential for drug developers. Indication of pathway activity may be derived from the detection of phosphorylated pathway members downstream of PI3K. In this application note, the levels of phospho-Akt are quantitated with Cisbio’s HTRF Cellular Assay kit.