Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA and Western Blotting

Western blotting is a traditional technique for detecting phosphorylated proteins within a variety of cell culture and tissue sample lysates. In recent years, new techniques that apply a sandwich antibody capture approach have become available for detecting phosphoproteins, such as Bio-Plex phosphoprotein assays. In this study, commercially available Bio-Plex phosphoprotein and total target assays, and phosphoprotein and total ELISA assays, are compared with western blotting in terms of sensitivity, detection range, and level of correlation, for the detection of phosphor- and total ERK and p38 MAPK.