Determination of Carbohydrates in Urine by HPAE-PAD

In this application note two high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) methods are compared for the analysis of urine samples. The first method, using a Thermo Scientific Dionex CarboPac PA20 Analytical Column minimizes eluent preparation with a Reagent-Free™ ion chromatography (RFIC™) eluent generator. The second method discussed takes advantage of the selectivity and high capacity of the Dionex CarboPac MA1 column. Both methods are shown to successfully determine carbohydrates in urine with good precision and minimal matrix effects from samples.