Determination of Catechins and Phenolic Acids in Red Wine by Solid Phase Extraction and HPLC

This application note demonstrates a simple and rapid HPLC method for the analysis of nine catechins and phenolic acids in red wine. Extraction of these polar analytes was achieved on Thermo Scientific™ HyperSep™ Retain PEP material. The polyphenols in the extracts were quantified by a matrix-standard calibration, with extracts from a wine sample spiked with increasing amounts of analytes. Determination of the polyphenols was performed by HPLC, using a Thermo Scientific Accucore™ PFP HPLC column under gradient mobile phase conditions.