Determination of Endogenous Cellular Kinase Activity Through the Use of an Automated, High Throughput AlphaScreen-Based Assay

In this application note from BioTek, the use of the AlphaScreen® SureFire® assay for the assessment of kinase signalling pathway modulation is described. The assay procedure was easily automated in low-volume 384-well format using the aspiration and non-contact dispensing capacilities of the EL406™ or MultiFlo™. The laser-based excitation and filter-based detection system of the Synergy™ NEO microplate reader was used to easily and efficiently quantify the emitted signal from the AlphaScreen® acceptor beads.