Determination of Enzyme Kinetics in the Eppendorf BioSpectrometer® kinetic Using a Hexokinase and Glucose-6-Phosphate-Dehydrogenase from <i>Saccharomyces cerevisiae</i>

This guide demonstrates two applications of measurements of enzyme kinetics using the Eppendorf BioSpectrometer kinetic; enzymatic determination of ATP concentration, and determination of enzyme activities. Learn how important parameters, such as optimal enzyme or substrate concentrations, measurement duration, linear range of the curve or reaction temperature, may be assessed in preliminary experiments from which activity measurements can then be based.