Determination of Km for ATP and Kemptide with Protein Kinase A Enzyme using Transcreener® ADP2 FP Assay

Transcreener® is a universal, high throughput biochemical assay based on detection of nucleotides, which are formed by thousands of cellular enzymes — many of which catalyze the covalent regulatory reactions that are central to cell signaling and are high value targets in drug discovery. This application note describes the use of the Transcreener® assay in the determination of Km for ATP and Kemptide with Protein Kinase A Enzyme.