Determination of Lipid Protein Binding Stoichiometry Using Microfluidic Diffusional Sizing

This application note assesses the stoichiometry of α-synuclein binding to lipid vesicles by microfluidic diffusional sizing (MDS) on the Fluidity One and Circular Dichroism (CD). We find that the results obtained are consistent between the two techniques, but that MDS presents advantages in both speed and simplicity.