Determination of multiple quality attributes of antibody-oligonucleotide conjugate with SEC-MALS and AEX-MALS

Multi-angle light scattering coupled to liquid chromatography (LC-MALS) can be used to analyze antibody-oligonucleotide conjugates (AOCs), providing a robust and straightforward approach to obtaining multiple quality attributes. Wyatt Technology demonstrates the successful application of LC-MALS with both size-exclusion chromatography (SEC) and anion exchange chromatography (AEX) to quantify the oligonucleotide-antibody ratio (OAR) and overall payload concentration of two AOC samples. Explore how through a single LC-MALS experiment, the complex molecular species present in the samples, was identified, including aggregates, fragments, and different OAR populations.