Determination of Nitidine Chloride, Toddalolactone, and Chelerythrine Chloride by HPLC

This application note describes the methodology used to develop an efficient and comprehensive HPLC method for the quality control analysis of Zanthoxylum nitidum (Roxb.) DC using the Thermo Scientific Dionex UltiMate™ 3000 HPLC System with Chromeleon™ CDS software. This dried root is used in Chinese herbal medicine as a cancer treatment, and is regulated by The Pharmacopeia of the People’s Republic of China (PPRC) 2010.