Determination of Nucleic Acid Concentrations Using Fluorescent Dyes in the Eppendorf BioSpectrometer® fluorescence

Fluorescence allows more sensitive and more specific determination of nucleic acids than the conventional method of UV-VIS spectroscopy. The present article describes quantification of dsDNA using the fluorescent dye PicoGreen®, with special focus on the preparation of samples for measurement in the Eppendorf BioSpectrometer, as well as the details of programming. The methods PicoGreen and PicoGreen-“short” are compared experimentally. Even though the method PicoGreen-“short” was measured using only 2 standards, high analytical accuracy was observed.