Determination of Thermodynamic Parameters ΔG and ΔH of a Small Molecule Binding to p38 using MST

In current drug discovery efforts, hits are discovered by conventional high-throughput or fragment screening campaigns. The only established technology that is capable of measuring the enthalpy of an interaction ΔH directly is ITC. The use of ITC early on in the drug discovery process is frequently hampered by its vast consumption of sample materials. This application note demonstrates that it is possible to gather this thermodynamic information by the use of MicroScale Thermophoresis (MST), taking full advantage of its unique benefits such as tiny sample consumption, and a temperature controlled sample mount.