Determining Protein Turnover in Fish with D7-Leucine

Moving from a static “snapshot” of a proteome to a dynamic view presents a considerable technical challenge, however, the utilization of stable isotope labeling of organisms in conjunction with mass spectrometry has led to considerable advances. These novel proteomic technologies have introduced the possibility of determining the turnover rates of multiple proteins in intact animal species including chicken and mice. This study extends the experimental strategy to measure the rates of synthesis and degradation of individual proteins in the skeletal muscle of fish.