Developing a Robust Self-Optimizing MS/MS QTOF Workflow for De Novo Verification of Synthetic Peptides in Biopharmaceutical Applications

This poster presents a method optimized QTOF MS system with self-adapting deep sequencing MS/MS capabilities (InstantExpertise™) that allows facile pharmaceutical product verification of synthetic peptides. Bioactive peptides are common, widely used supplement products in pharma, biopharma, as well as nutritional industries. The synthesis of bioactive peptides with accurate amino acid sequences is pivotal to ensure correct biological activity; thus, methods that ensure accurate de novo verification as well as simultaneous impurity characterization are very important. This method combines high speed self-optimized MS/MS, accurate isotopic patterns, and high mass accuracy fragment data.