Developing an LC-MS/MS Assay for Screening Tracheal Aspirate Samples for Pepsin

A gastric reflux event (GRE) is defined as the passing of the gastroduodenal contents into the oesophagus and causing damage to the oesophagal mucosa. Monitoring GREs can aid clinical studies for drug development and inform treatment regimes in the clinic to minimise GRE occurrence, which can dramatically improve quality of life. This application note presents the development of a robust, semi-quantitative LC-MS/MS method to detect pepsin in tracheal aspirate using a Waters Acquity hyphenated to a Waters Xevo TQS.