Developing Assays for Screening GPCRs with Transiently Transfected Cells Using the MaxCyte® STX™ System

This application note demonstrates that the MaxCyte STX transient transfection system enables rapid development of cell-based assays to screen GPCRs and other target molecules. Cells transfected with the MaxCyte STX system are suitable for screening with a variety of automated assay technologies, such as the FLIPRTETRA®. MaxCyte STX users can exert tight control over assay sensitivity and dynamic range by varying the concentration of plasmid DNA in the electroporation reaction.