Developing hydrophilic interaction liquid chromatography (HILIC) separation methods with HALO HILIC and HALO Penta-HILIC columns

Method developers rely on reversed-phase (RP) conditions for most of their separations. However, what happens when there are polar analytes that are not well retained under reversed-phase conditions? One option is to use hydrophilic interaction liquid chromatography (HILIC). In this whitepaper, Advanced Materials Technology provides experimental data and helpful information on the development of HILIC separation methods using the HALO^® HILIC and HALO^® Penta-HILIC columns.