Development of a Highly-Sensitive Multiplex Assay Using Monoclonal Antibodies for the Measurement of Kappa and Lambda Free Light Chains in Serum and Urine

Despite the enormous advantages offered by commercial assays to measure κ and λ immunoglobulin free light chains (FLC), a number of analytical limitations can be attributed to the use of sheep polyclonal antisera as capture antibodies. A second generation test using monoclonal antibodies (mAbs) is desirable. This poster describes a highly-sensitive multiplex assay which uses mAbs for the simultaneous measurement of FLC's in urine and serum.