Development of a Medium Throughput Electrophysiology Assay to Investigate State-Dependency of Sodium Channel Blockers Using QPatch

This application note evaluates the suitability of the CHO ChanClone™ Na_v1.5 cell line, over-expressing recombinant human Na_v1.5 (a voltage-gated sodiumchannel), for use on the automated electrophysiology platform, QPatch. The researchers develop an assay suitable for the investigation of pharmacological modulators of Na_v1.5 channels. The automated electrophysiology platform is capable of producing data of equivalent quality to that obtained using manual patch, but at significantly higher throughput (up to 16 cells can be patched in parallel).