Development of a Multiplex High Throughput Screening Assay Cascade for Evaluating Receptor Tyrosine Kinase Binding and Activation

Screening for protein based therapeutics that inhibit ligand binding and/or downstream activation events in living cells represents a powerful approach for the discovery of novel drugs. This scientific poster, presented at ELRIG Drug Discovery 2012, describes how IntelliCyt have developed a series of live-cell based screening assays for the HTFC® Screening System that addresses multiple steps in these ligand-receptor driven pathways. For these studies, interactions between EphA2 receptor and its ligands were used as a model system. The results demonstrate that high throughput flow cytometry can be leveraged to improve productivity in the drug discovery process.