Development of a New Real-Time PCR Quantitation System for Human and Y DNA Analysis and Normalization Software

This application note discusses the development of a new technology for real-time quantitative PCR. The technology offers an advantage over currently available systems by simultaneously quantitating both the total human DNA and male-specific DNA within a sample, in addition to an internal PCR control. Simultaneous quantification of autosomal and Y-chromosome DNA means: less variability, less time and more valuable data.