Development of a No-Wash 4-Plex Apoptosis Screening Kit and Validation for Phenotypic Screening

In this application note, Intellicyt reports on the development of a 4-plex apoptosis screening assay in a no-wash format. Validation of the assay performance using Jurkat as the cell model yielded z’ values of > 0.8 for all endpoints, demonstrating broad applicability of the assay for phenotypic screening campaigns as well as toxicity profiling.