Development of a twin-column continuous chromatography oligo purification process

Peptide and Oligo synthesis technologies are well developed for large scale manufacturing of pharmaceutical materials. Isolation and purification is a critical step in these manufacturing processes, and almost always utilizes chromatography. Multi-column counter-current solvent gradient purification (MCSGP) is a twin column continuous chromatography process that has significantly more capability than a traditional single-step batch process. In this poster, YMC America shows that the product yield is greatly improved with MCSGP while avoiding many complications encountered with a batch process.