Development of an All-Recombinant Intact Protein Standard for LC & MS Application Development & System Suitability Testing

This application note describes the development of a multi-purpose intact protein standard for LC, LC-MS, and LC-MS/MS quality control and application development. Quality and stability of the selected proteins were verified by SDS-PAGE, UV HPLC, infusion-MS and LC-MS, and MS/MS and top-down data was analysed with Thermo Fisher Scientific software packages. The method is applicable to proteins with a wide MW range, m/z range and is compatible for clinical research applications.