Development of an In Vitro Pre-clinical Cellular Assay to Predict

This poster describes the development of an in vitro assay to evaluate biologics for induction of cytokine storm. Frozen human and NHP PBMCs are cultured with test articles and assayed for cytokine production and cell proliferation. Anti-CD28 superagonist antibodies, which have been shown to be similar in mechanism as TGN14124, are used as a positive control. The study confirms the necessity of testing novel antibody agents in different in vitro testing approaches.