Development of Ultra-Fast pH-Gradient Ion Exchange Chromatography for the Separation of Monoclonal Antibody Charge Variants

The pre-formulated buffers for pH gradient, introduced by Thermo Fisher Scientific, have greatly simplified the development of ion exchange chromatography (IEX) of monoclonal antibodies (mAbs). This application note shows how to push the throughput of pH gradient IEX even further. To achieve this, a MAbPac SCX-10 RS column, 5 μm, 2.1 × 50 mm was operated on a new Thermo Scientific™ Vanquish™ UHPLC system. The Vanquish UHPLC is a fully biocompatible system, suitable for the analysis of intact proteins.