Differential Extraction of Mixtures in Sexual Assault Casework Using Pressure Cycling Technology (PCT)

Conventional differential extraction methods used for the separation of DNA from sperm cells and epithelial cells in sexual assault casework rely on a complicated procedure of selective digestion
separation of epithelial cell fractions followed by sperm cell lysis to generate the male genetic profile.
The objective of this study is to develop a selective pressure DNA extraction procedure that is designed to burst open and extract DNA from male sperm cells while leaving the excess female cells rape kits or other mixed forensic stains unaffected.