Direct Analysis of Opioids and Metabolites from Whole Blood Using Ostro Sample Preparation Plates Combined with UPLC-MS/MS for Forensic Toxicology

The analysis of natural and synthetic opioid drugs continues to be an important aspect of forensic toxicology. Many sample preparation strategies have been used for whole blood analysis; one of the simplest involves cell lysis followed by protein precipitation. The method presented in this study describes a rapid and straightforward sample preparation strategy using Ostro Sample Preparation Plates whereby whole blood samples can be pre-treated to lyse the cells, precipitated with acetonitrile, and eluted using a simple 96-well format.