Direct Analysis of Opioids and Metabolites in Oral Fluid by Mixed-Mode μElution SPE Combined with UPLC-MS/MS for Forensic Toxicology

This application highlights a method for the analysis of 26 opioid drugs and metabolites by mixed-mode SPE followed by UPLC®-MS/MS. Glucuronide metabolites are directly analyzed, eliminating the need for enzymatic or chemical hydrolysis.