Direct Analysis of Urinary Opioids and Metabolites by Mixed-Mode μElution SPE Combined with UPLC/MS/MS for Forensic Toxicology

This application note highlights a method for the analysis of 26 opioid drugs and metabolites by mixed-mode SPE followed by UPLC®/MS/MS. Glucuronide metabolites are directly analyzed, eliminating the need for enzymatic or chemical hydrolysis. Direct comparison demonstrates that mixed-mode SPE has improved linearity, greater accuracy and precision, and fewer matrix effects than a simple dilute and shoot method. Previously confirmed, incurred samples were also analyzed, allowing for additional evaluation of this method.