Direct Detection of Glutathione Conjugates in Human Liver Microsomes Using ACQUITY UPLC I-Class, Xevo G2-S QTof, and UNIFI

This application note demonstrates the use of MSE data acquisition for detecting reactive metabolites using clozapine as a model compound. Through integrated charge state deconvolution and detection using the Metabolite Identification Application Solution in UNIFI, all metabolites, including parent, phase I metabolites, and phase II GSH conjugates are detected and tracked across the dataset automatically.