Direct Separations of the Six Positional Isomers of Disubstituted Benzoic Acids Using ACQUITY UPC2 Torus Columns

This application note illustrates the rapid and efficient separation of all six of the possible positional isomers of both the six disubstituted dimethoxybenzoic acids and also the six dimethylbenzoic acids. This latter example is particularly interesting, as DMBA is a metabolite of trimethylbenzene and its appearance in human urine can be used as a clinical marker for measuring industrial exposure to TMB.