DNA Extraction from Normal and Cancer Tissues in a Section on a Histology Slide

Loss of heterozygosity (LOH) in colon cancer tissue was assessed by PCR using microsatellite markers. DNA was extracted by DEXPAT™ from normal tissue and from neoplastic lesions of formalin-fixed, paraffin-embedded colorectal tumors. To achieve accurate and highly sensitive PCR amplification, DNA extracted with DEXPAT™ is best used in conjunction with TaKaRa Ex Taq™.