Domoic acid & amino acids in algae extracts analyzed with LCMS

This LCMS method can achieve very low detection levels of domoic acid and amino acids without the need for analyte derivatization. Figures A and B show the chromatograms of both algae extracts. Figure C shows a zoom-in overlay of both cultures 1 and 2 for the histidine peak. This application note shows how the tryptophan levels are significantly different between the two cultures whereas the other levels are comparable. It also produces sufficient separation of domoic acid from tryptophan, which is often an interferent in domoic acid quantitation.