Duolink® PLA Probemaker Guide

This application note describes the use of the Duolink^® In Situ Probemaker for the conjugation of PLA oligonucleotides (PLUS or MINUS) to any antibody for use in Duolink^® PLA experiments. Probemaker-generated PLA antibodies can be used in combination with or independent from standard Duolink^® PLA Probes, as long as a PLUS PLA probe and a MINUS PLA probe are used.