Dye‐Protein Binding Monitored in a Microliter Volume Using Time-Resolved Fluorescence

The DeltaFlex system, with the Microsense cuvette, can perform time‐resolved fluorescence to obtain kinetic information on processes occurring on the millisecond time scale in microliter volumes. In this application note, the interaction of a dye binding to a protein was monitored. This was achieved by the rapid and efficient data-acquisition enabled by the 100 MHz excitation rate, coupled with the low dead time of the timing electronics. The use of time‐resolved fluorescence enables a fuller picture to be obtained, by providing a further fluorescence parameter (the fluorescence lifetime) with the advantage that this is also independent of concentration.