Easy perfusion for anchorage-dependent cell culture

Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vaccine production. In perfusion bioprocesses it is possible to constantly add nutrients and to remove byproducts, while retaining the cells in the bioreactor. In this application note, Eppendorf demonstrates how the BioFlo 320 vessel equipped with a spin filter for microcarrier can be used as a platform for high-density Vero cell culture, suitable for vaccine production.