Effective Site-Specific Isotopic Labeling and Expression Optimization Using BioExpress® 2000 Media

Recombinant genes are often used to generate protein reagents as potential targets for new small-molecule drugs. Labeling amino acids during this process elucidates the structure of these proteins and any drug interactions during NMR studies. This application note explores the expression and labeling of histidine in human serine/threonine kinase in the Spodoptera frugiperda (SF9) cell line.