Effectiveness of CRISPR-Cas9 Using Pools of Synthetic crRNAs in High-Content Analysis Screening Experiments

Gene knockout using the CRISPR-Cas9 system has emerged as a powerful technology for loss-of-function screening. Although screening using pooled lentiviral sgRNA libraries is a powerful way of discovering gene function arrayed screening expands the types of phenotypic readouts from simple population enrichment or depletion to complex multiparametric high-content imaging and morphological assays. Chemical synthesis of guide RNAs for CRISPR-Cas9 gene editing allows for accurate and rapid production of CRISPR libraries and enables screening in an arrayed, one-gene-per-well fashion.